commercial hepatocyte maintenance medium  (ZenBio)

Journal: Current Research in Toxicology

Article Title: Microphysiological system to address the opioid crisis: A novel multi-organ model of acute opioid overdose and recovery

doi: 10.1016/j.crtox.2024.100209

Figure Lengend Snippet: Immunocytochemical characterization of opioid receptor expression of the multi-organ system components under flow condition. A) opioid receptor expression of cardiomyocytes, B) opioid receptor expression of hepatocytes, C) opioid receptor expression of skeletal muscle, D) opioid receptor expression of preBötC neurons; scale bars = 100um.

Article Snippet: Following an additional 2 h incubation, plating medium was removed, and the wells were filled with 1 mL of hepatocyte maintenance medium (Novabiosis, NHMM).

Techniques: Expressing

Journal: Current Research in Toxicology

Article Title: Microphysiological system to address the opioid crisis: A novel multi-organ model of acute opioid overdose and recovery

doi: 10.1016/j.crtox.2024.100209

Figure Lengend Snippet: Phase images of organ components inside the multi-organ system. A) hepatocytes after assembly into the multi-organ system, B) cardiomyocyte cantilevers after assembly into multi-organ system, C) skeletal muscle cantilevers after assembly into the multi-organ system, D) patterned cardiomyocyte MEA after assembly into the multi-organ system, E) patterned preBötC MEA after assembly into the multi-organ system; scale bars = 50um.

Article Snippet: Following an additional 2 h incubation, plating medium was removed, and the wells were filled with 1 mL of hepatocyte maintenance medium (Novabiosis, NHMM).

Techniques:

Journal: Current Research in Toxicology

Article Title: Microphysiological system to address the opioid crisis: A novel multi-organ model of acute opioid overdose and recovery

doi: 10.1016/j.crtox.2024.100209

Figure Lengend Snippet: Skeletal muscle contractile force and hepatocyte enzymatic activity. A) skeletal muscle amplitude on cantilevers, B) no significant change was observed in hepatocyte enzymatic activity; data collected from more than 3 independent biological replicates per condition, data is shown as mean with error bars indicating SEM.

Article Snippet: Following an additional 2 h incubation, plating medium was removed, and the wells were filled with 1 mL of hepatocyte maintenance medium (Novabiosis, NHMM).

Techniques: Activity Assay

Journal: Current Research in Toxicology

Article Title: Microphysiological system to address the opioid crisis: A novel multi-organ model of acute opioid overdose and recovery

doi: 10.1016/j.crtox.2024.100209

Figure Lengend Snippet: Viability assays. A) Cardiac, SKM, and preBötC viability determined via Alamar Blue, B) hepatocyte viability determined via MTT (control: gray, methadone: orange, naloxone: green, methadone + naloxone: blue). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).

Article Snippet: Following an additional 2 h incubation, plating medium was removed, and the wells were filled with 1 mL of hepatocyte maintenance medium (Novabiosis, NHMM).

Techniques: Control